RNA: primed for packing?

نویسندگان

  • Katrin Karbstein
  • Jennifer A Doudna
چکیده

How does RNA's seemingly subtle 2'-hydroxyl group contribute to its diverse biological functions? Using quantitative structure-activity relationship (QSAR) analysis, Piccirilli and colleagues find that the 2'-OH fosters van der Waals and hydrophobic interactions that play underappreciated roles in RNA structure and molecular recognition.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Calculation of standard atomic volumes for RNA and comparison with proteins: RNA is packed more tightly.

Traditionally, for biomolecular packing calculations research has focused on proteins. Besides proteins, RNA is the other large biomolecule that has tertiary structure interactions and complex packing. No one has yet quantitatively investigated RNA packing or compared its packing to that of proteins because, until recently, there were no large RNA structures. Here we address this question in de...

متن کامل

Protein-primed and de novo initiation of RNA synthesis by norovirus 3Dpol.

Noroviruses (Caliciviridae) are RNA viruses with a single-stranded, positive-oriented polyadenylated genome. To date, little is known about the replication strategy of norovirus, a so-far noncultivable virus. We have examined the initiation of replication of the norovirus genome in vitro, using the active norovirus RNA-dependent RNA polymerase (3D(pol)), homopolymeric templates, and synthetic s...

متن کامل

Calculation of Standard Atomic Volumes for RNA Cores and Comparison with Proteins: RNA is packed more tightly than Protein

YJMBI 56900—8/12/2004—15:52—SFORSTER 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 TE D PR OO F Traditionally, for biomolecular packing calculations research has focused on proteins. Besides proteins, RNA is the other large biomolecule that has tertiary structure interactions and complex packing. No one has yet quantitatively investigated RNA packing nor compared i...

متن کامل

Human Immunodeficiency Virus Reverse Transcriptase

3’-Azido-3’-deoxythymidine 5’4riphosphate (AZTTP) was an efficient substrate for the human immunodeficiency virus 1 reverse transcriptase. It was incorporated into both homopolymer and defined sequence DNA-primed RNA templates and DNA-primed DNA templates. The substrate and inhibitor kinetics of both AZTTP and dTTP were dependent on the template-primer and reaction conditions used. dTMP was inc...

متن کامل

Protein-Primed and De Novo Initiation of RNA Synthesis by Norovirus 3D

Noroviruses (Caliciviridae) are RNA viruses with a single-stranded, positive-oriented polyadenylated genome. To date, little is known about the replication strategy of norovirus, a so-far noncultivable virus. We have examined the initiation of replication of the norovirus genome in vitro, using the active norovirus RNAdependent RNA polymerase (3D), homopolymeric templates, and synthetic subgeno...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Chemistry & biology

دوره 11 2  شماره 

صفحات  -

تاریخ انتشار 2004